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Image Search Results
Journal: bioRxiv
Article Title: The restricted nature of protein glycosylation in the mammalian brain
doi: 10.1101/2020.10.01.322537
Figure Lengend Snippet: Protein lysate from mouse cortex and cerebellum with human plasma as a positive control was treated with or without PNGase F and visualized using biotinylated lectins (Con A, GNL, PHA-E, AAL, RCA, and SNA) in addition to immunoblotting for actin and staining for total protein. Non-specific binding of lectins to PNGase F is noted by an asterisk (*) near 35 kDa. A schematic with common lectin-binding sites is shown for reference.
Article Snippet: Membranes were then incubated in 5% BSA in TBS-Tween 0.1% for 1 hour, followed by incubation with
Techniques: Positive Control, Western Blot, Staining, Binding Assay